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fluorescent biosensors for calcium  (Jackson Laboratory)

 
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    Structured Review

    Jackson Laboratory fluorescent biosensors for calcium
    Gpr120 mRNA was predominantly detected in mouse islet δ cells and some α and β cells . (A–F) gpr120 (green) and sst (A and B), glucagon (gcg; C and D), or insulin (ins; E and F) (magenta) mRNA were detected in adult mouse pancreatic cryosections by <t>fluorescent</t> in situ hybridization. Representative sections are shown at 20× (A, C, and E) and 63× (B, D, and F) magnification. DAPI (blue). Scale bar = 10 μm. (G) Normalized browser plot illustrating gpr120 mRNA expression in adult mouse islet cells based on transcriptomic analyses of FACS-sorted α, β, and δ cells from reporter lines described in . (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
    Fluorescent Biosensors For Calcium, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fluorescent+biosensors+for+calcium/calcium+gcamp6s+indicator/pmc07873385-65-8-21
    Average 86 stars, based on 1 article reviews
    fluorescent biosensors for calcium - by Bioz Stars, 2026-09
    86/100 stars

    Images

    1) Product Images from "Free fatty acid receptor 4 inhibitory signaling in delta cells regulates islet hormone secretion in mice"

    Article Title: Free fatty acid receptor 4 inhibitory signaling in delta cells regulates islet hormone secretion in mice

    Journal: Molecular Metabolism

    doi: 10.1016/j.molmet.2021.101166

    Gpr120 mRNA was predominantly detected in mouse islet δ cells and some α and β cells . (A–F) gpr120 (green) and sst (A and B), glucagon (gcg; C and D), or insulin (ins; E and F) (magenta) mRNA were detected in adult mouse pancreatic cryosections by fluorescent in situ hybridization. Representative sections are shown at 20× (A, C, and E) and 63× (B, D, and F) magnification. DAPI (blue). Scale bar = 10 μm. (G) Normalized browser plot illustrating gpr120 mRNA expression in adult mouse islet cells based on transcriptomic analyses of FACS-sorted α, β, and δ cells from reporter lines described in . (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
    Figure Legend Snippet: Gpr120 mRNA was predominantly detected in mouse islet δ cells and some α and β cells . (A–F) gpr120 (green) and sst (A and B), glucagon (gcg; C and D), or insulin (ins; E and F) (magenta) mRNA were detected in adult mouse pancreatic cryosections by fluorescent in situ hybridization. Representative sections are shown at 20× (A, C, and E) and 63× (B, D, and F) magnification. DAPI (blue). Scale bar = 10 μm. (G) Normalized browser plot illustrating gpr120 mRNA expression in adult mouse islet cells based on transcriptomic analyses of FACS-sorted α, β, and δ cells from reporter lines described in . (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Techniques Used: In Situ Hybridization, Expressing

    Related Articles

    In Situ Hybridization:

    Article Title: Free fatty acid receptor 4 inhibitory signaling in delta cells regulates islet hormone secretion in mice
    Article Snippet: We used the following Cre drivers to express fluorescent biosensors for calcium (GCaMP6s; Gt(ROSA)26Sor tm96(CAG−GCaMP6s)Hze ) [ ] or cAMP ( https://www.jax.org/strain/032205 ) (cAMPER, Jackson Laboratory, strain #032205) [ ] directly and specifically in α cells ( https://www.jax.org/strain/030346 ) [ ], β cells (B6.FVB(Cg)-Tg(Ucn3-cre)KF43Gsat/Mmucd) [ ], or δ cells (B6N.Cg-Ssttm2.1(cre)Zjh/J).

    Expressing:

    Article Title: Free fatty acid receptor 4 inhibitory signaling in delta cells regulates islet hormone secretion in mice
    Article Snippet: We used the following Cre drivers to express fluorescent biosensors for calcium (GCaMP6s; Gt(ROSA)26Sor tm96(CAG−GCaMP6s)Hze ) [ ] or cAMP ( https://www.jax.org/strain/032205 ) (cAMPER, Jackson Laboratory, strain #032205) [ ] directly and specifically in α cells ( https://www.jax.org/strain/030346 ) [ ], β cells (B6.FVB(Cg)-Tg(Ucn3-cre)KF43Gsat/Mmucd) [ ], or δ cells (B6N.Cg-Ssttm2.1(cre)Zjh/J).



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    Gpr120 mRNA was predominantly detected in mouse islet δ cells and some α and β cells . (A–F) gpr120 (green) and sst (A and B), glucagon (gcg; C and D), or insulin (ins; E and F) (magenta) mRNA were detected in adult mouse pancreatic cryosections by <t>fluorescent</t> in situ hybridization. Representative sections are shown at 20× (A, C, and E) and 63× (B, D, and F) magnification. DAPI (blue). Scale bar = 10 μm. (G) Normalized browser plot illustrating gpr120 mRNA expression in adult mouse islet cells based on transcriptomic analyses of FACS-sorted α, β, and δ cells from reporter lines described in . (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
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    Image Search Results


    Gpr120 mRNA was predominantly detected in mouse islet δ cells and some α and β cells . (A–F) gpr120 (green) and sst (A and B), glucagon (gcg; C and D), or insulin (ins; E and F) (magenta) mRNA were detected in adult mouse pancreatic cryosections by fluorescent in situ hybridization. Representative sections are shown at 20× (A, C, and E) and 63× (B, D, and F) magnification. DAPI (blue). Scale bar = 10 μm. (G) Normalized browser plot illustrating gpr120 mRNA expression in adult mouse islet cells based on transcriptomic analyses of FACS-sorted α, β, and δ cells from reporter lines described in . (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Journal: Molecular Metabolism

    Article Title: Free fatty acid receptor 4 inhibitory signaling in delta cells regulates islet hormone secretion in mice

    doi: 10.1016/j.molmet.2021.101166

    Figure Lengend Snippet: Gpr120 mRNA was predominantly detected in mouse islet δ cells and some α and β cells . (A–F) gpr120 (green) and sst (A and B), glucagon (gcg; C and D), or insulin (ins; E and F) (magenta) mRNA were detected in adult mouse pancreatic cryosections by fluorescent in situ hybridization. Representative sections are shown at 20× (A, C, and E) and 63× (B, D, and F) magnification. DAPI (blue). Scale bar = 10 μm. (G) Normalized browser plot illustrating gpr120 mRNA expression in adult mouse islet cells based on transcriptomic analyses of FACS-sorted α, β, and δ cells from reporter lines described in . (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Article Snippet: We used the following Cre drivers to express fluorescent biosensors for calcium (GCaMP6s; Gt(ROSA)26Sor tm96(CAG−GCaMP6s)Hze ) [ ] or cAMP ( https://www.jax.org/strain/032205 ) (cAMPER, Jackson Laboratory, strain #032205) [ ] directly and specifically in α cells ( https://www.jax.org/strain/030346 ) [ ], β cells (B6.FVB(Cg)-Tg(Ucn3-cre)KF43Gsat/Mmucd) [ ], or δ cells (B6N.Cg-Ssttm2.1(cre)Zjh/J).

    Techniques: In Situ Hybridization, Expressing